Assess qPCR Efficiency Using a 10-Fold Serial Dilution
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YouTubeAddgene
Assess qPCR Efficiency Using a 10-Fold Serial Dilution
To optimize your qPCR conditions, try varying conditions such as annealing temperature or primer concentration Then use a 10-fold serial dilution to check the efficiency of your qPCR. An example of this can be found in our AAV Titration by qPCR protocol: https://www.addgene.org/protocols/aav-titration-qpcr-using-sybr-green-technology/ https ...
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QPCR Basics
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