Comparative genomic hybridization (CGH) was developed to identify pathogenic DNA copy-number changes (e.g., duplications, deletions) on a genome-wide scale, and to map these changes to genomic ...

PCR primers were designed in an average distance of 0.7 kb upstream and downstream of each predicted junction and amplifications were performed using standard protocols of Promega Master Mix (Promega ...

Early efforts to examine genomic changes in the clinical setting relied on cytogenetic techniques such as chromosome karyotyping, a widely used approach to examine chromosomes and identify changes ...

Figure 1: CGH analyses of genome copy number in murine tissues, tumors and cell lines. We assessed the influence of normal or unaffected cells on our ability to detect single-copy number changes by ...